glutathione stransferase tag Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused protein glutathione S-transferase

10.1016/j.heliyon.2019.e02645 [DOI] [PMC free article] [PubMed] [Google Scholar] Farhadnejad H., Asghari G., Mirmiran P., Mirmiran P., Yuzbashian E., Azizi F

Tumour tissues (100500 mg) were dissected into smaller fragments to cover the filter surface of 0.22 m nylon-filtered microcentrifuge tubes (Corning, 8160), taking care to minimize mechanical cell lysis

Although mechanistic studies are needed to confirm that Nrf2-mediated responses are at play when -synuclein is overexpressed in our model, Lastres-Becker et al

Ferroptosis can be delayed by pretreatment with nutlin-3 (an inhibitor of MDM2), suggesting that stable and persistent p53 reduces the rate of ferroptosis (Ji et al., 2021)

However, the precise mechanisms underlying their crosstalk remain unclear