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10.1371/journal.pone.0117807 178 McCannM

Uneven tone starts to balance out

Protein expression and purification For the expression of C-terminally PA-tagged HGF proteins, vectors coding for tcHGF-PA or scHGF(Xa)-PA were transfected into HEK293T cells using X-tremeGENE HP DNA Transfection Reagent (Roche) and cultured in a serum-containing medium