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Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

Like any injection, there is some discomfort during the treatment, but it is minimal

Not for direct personal use

The most severe and potentially dangerous interaction occurs with nitroglycerin and other nitrate-based blood pressure or angina medications