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Multimed Tools Appl 75:34253451

Also, topical application to the eye has resulted in blue discoloration of the eye and surrounding tissues that can be permanent

To initiate the differentiation process, differentiation medium consisting of DMEM/F12 (Sigma-Aldrich) with 1% N2 supplement (Thermo Fisher Scientific), 1 g/mL tetracycline (Sigma-Aldrich), 0.49 mg/mL dibutyryl-cyclic adenosine monophosphate (Sigma-Aldrich), and 2 ng/L glial cell line-derived neurotrophic factor (R&D Systems) was used [53]