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scRNA-seq was introduced for revealing the diversity of CAFs in CRC tumor microenvironment (TME), where fibroblast specific protein 1 (FSP1) was used to enrich fibroblast-derived cell subset prior to sequencing so as to specifically acquire and analyze fibroblast-derived cell accurately without interference from other types of cells

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For the CT, the energy and macronutrient intake were adjusted with the food of the habitual diet