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Through chemogenetic activation of astrocytes calcium activity in the BF, astrocytes decreased the consolidation of NREM sleep by amplifying the activation of the GABAergic neurons, which are more active during wakefulness than during sleep (Hassani et al., 2009), increasing persistent inhibition in the BF neural network

The resulting cells were harvested through centrifugation at 800 rpm for 5 min and then washed twice with ice-cold phosphate-buffered saline (PBS)

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Saponins another highly active phytochemicals considered to increase fecal cholesterol excretion (Matsui et al

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