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Circular dichroism spectroscopy, which we did to understand the secondary structure of our best candidate bovine IF, which indicated a predominantly helical secondary structure consistent with native folding, while computational docking predicted bovine and human IF to have optimal geometric complementarity and stabilising energies with B12, in agreement with our experimental binding data

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Record Keeping Documenting your protocol details, product information, and observed results over time provides valuable data for optimizing future cycles