glutathione affinity chromatography In-house preparation of hydrogels for batch purification of S-transferase tagged recombinant proteins | BMC Biotechnology including many known carcinogens and mutagens GST-Tagged Protein Purification

doi: 10.1186/s12939-019-0918-1

Current Pharmaceutical Design, 16(10), 12241234

Key Points Individuals with GSTM1 -positive and GSTT1 -positive genotypes are at increased risk for renal cell carcinoma (RCC) when occupationally exposed to certain toxicants Individuals with the GSTM1 -null genotype are at increased risk for bladder cancer, which might be potentiated by smoking and occupational hazards Data on the influence of GSTT1 polymorphism on the risk for transitional cell carcinoma (TCC) are inconsistent In the process of kidney cancerization, expression of GSTA isoenzymes is downregulated, which contributes to decreased antioxidant capacity in RCC and might enhance RCC growth In the malignant phenotype of TCC of the urinary bladder, upregulation of GSTT1 and GSTP1 might provide a reduced cellular environment and inhibit apoptotic pathways, respectively

A proper evaluation is important before starting any treatment or supplement plan

Publication and enthusiasm bias Finally, much of the accessible GHK-Cu literature is authored by a small number of closely associated researchers and enthusiasts, and negative or null results are less likely to be published or promoted