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Assembly of the three PCR products two from both flanking regions of the target gene, one from the antibiotic resistance gene was accomplished using overlap extension PCR with primers hybridizing at both extreme ends, and the resulting DNA fragment was purified by agarose gel electrophoresis

Our latest article explores the journey of the naturally occurring plant polyphenols found in Metabolaidfrom digestion and interaction with the gut microbiome to absorption and the cellular signalling pathways researchers continue to investigate