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The cDNA libraries were prepared using the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, USA), and qRT-PCR was performed using Power SYBR Green PCR Master Mix (Life Technologies, USA) on a Quant Studio 5 real-time PCR machine (Life Technologies, USA) to determine relative fold changes in target gene expression

Pairwise comparisons using Average Annual Percent Change (AAPC) were also performed between three outcomes

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Research from the National Cancer Institute has documented several potential mechanisms by which IV vitamin C may affect cancer cells: Phase I clinical trials at medical institutions including the University of Iowa and Thomas Jefferson University have demonstrated safety and potential benefits when HDIVC is administered alongside standard chemotherapy for various cancer types

As iodixanol is available as a sterile solution of density 1.32 g/ml (OptiPrep) it considerably simplifies gradient solution preparation