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Oxidative Stress Reduction: Helps neutralise free radicals and protect cells from damage

pp 99103

CHAC1 degrades GSH, leading to GSH depletion and oxidative stress

Neuro Endocrinol Lett 32:13340, PMID: 21552194 Kaddurah-Daouk R, Yuan P, Boyle SH, Matson W, Wang Z, Zeng ZB, Zhu H, Dougherty GG, Yao JK, Chen G, Guitart X, Carlson PJ, Neumeister A, Zarate C, Krishnan RR, Manji HK, Drevets W (2012) Cerebrospinal fluid metabolome in mood disorders-remission state has a unique metabolic profile

In vitro acetylation assay For the in vitro acetylation assay, 2 g of purified His-SUMO-ACAT1 enzyme was incubated with 2 g of substrate (either GST-DLD WT or K430R) in a 50 L reaction