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The animals were euthanized using carbon dioxide gas at approximately 48 h post-inoculation, and the liver, colon tissue and cecal contents were collected for further processing

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Subsequently, 20 L of GSH (final concentration of 5.0 mM) was added to the wells

If you plan on taking 1000 mg of glutathione for many months, it may be worth monitoring your zinc intake or speaking with a healthcare provider about a balanced routine