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Native MS preserves non-covalent interactions and allows quantification of tight homo-oligomer dissociation constants without reverting to chaotropic unfolding as well as the ability to resolve differentially modified protein species 16,30

Chest 128 , 16111619 (2005)

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Finally, the membranes were washed with PBST three times, and a ChemiDoc XRS System (Bio-Rad, CA, USA) was used to detect the expression of proteins with an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China)