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For PAS staining to evaluate glycogen and basement membranes, tissue sections were oxidized with periodic acid (C0142S, Beyotime Biotechnology, China), treated with Schiffs reagent (C0142S, Beyotime Biotechnology, China), and counterstained with Hematoxylin (Bry-0001-01, Runner bio, China)

Sections were washed twice in TBST, followed by detection of secondary antibody using chromogen substrate buffer DAB+ (#K3468, Dako North America, Inc., Carpinteria, CA, USA) and DAB-chromogen (#K3468, Dako North America, Inc., Carpinteria, CA, USA)

Isomalt is an equimolar mixture of two mutually diastereomeric disaccharides, each composed of two sugars: glucose and mannitol --D-glucopyranosido-16-mannitol- and also glucose and sorbitol --D-glucopyranosido-16-sorbitol-

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Mahanimbine, a carbazole alkaloid found in curry leaves, has been observed to enhance lipid breakdown and inhibit fat accumulation in adipocytes, the cells responsible for storing fat in the body