The most remarkable instance is in selenoprotein F (SELENOF), an ER protein involved in glycoprotein folding quality control
Moreover, the acidic assay conditions (pH 3.6) can precipitate proteins (e.g., milk casein) and generate pro-oxidant Fe 2+ ions, which may propagate radical chain reactions (Pulido et al., 2000
Experience shows that three focused actionssoothing the stomach to halt vomiting, regulating bowels to shorten diarrhea, and replacing fluids before dehydration snowballsturn a multi-day ordeal into a brief glitch
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In case of ethyl methanesulfonate mutants, the region surrounding the expected point mutation was amplified by PCR and the single point mutation was verified by sequencing
