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Afterward, the membranes were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and immunoreactive bands were detected by a Fluorchem Imaging System upon staining with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA)

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While all those data indicate that MOR phosphorylation would play a crucial role in desensitization, Qiu and collaborators showed that a truncated mutant of MOR from S363 is able to undergo a similar desensitization to the wild type demonstrating that receptor phosphorylation is not an absolute prerequisite for desensitization (Qiu et al., 2003)

Two of the studies in which incretin hormones were evaluated showed a significant increase in GLP-1 after 8 weeks of high-dose glutamine supplementation

This limitation highlights the critical importance of early intervention during the reversible disease phase