28 gauge needle melanotan gauge, Metal Hub (N), PN: 90028 51 mm, Point Style 2: Sharp, Beveled, Curved, 6 per Pack EMG Needle Electrode Ambu® Neuroline

Gel filtration chromatography Recombinant PGRMC1 (a.a.44195) (10 g) wt, Y113F or C129S mutant, treated with 5 mmol l 1 sodium dithionite and/or CO gas or left untreated, was separated on a Superdex 200 column equilibrated in buffer containing 20 mmol l 1 Tris-HCl (pH 7.5) and 100 mmol l 1 NaCl using a SMART system (GE Healthcare)

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doi: 10.1038/s41569-023-00957-0 7 DixonSJLembergKMLamprechtMRSkoutaRZaitsevEMGleasonCEet al

Determination of Blood APAP Concentrations by Gas ChromatographyMass Spectrometry At the indicated time intervals after APAP administration (200 mg/kg), the blood samples were collected from both strain mice

The selected amino acid site in the nanobody was replaced with a UAG stop codon by site-directed mutagenesis for incorporating ncAA with corresponding primers (Table S5)