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Generally, the tissue distribution and the persistence of expression of lipoplex and polyplex systems is mainly dependent, like with small molecular drugs following parenteral administration, also on the pharmacokinetics (clearance, distribution volume), the formulation (size, charge, PEGylation, etc.) and the dosage regimen (high volume bolus, sequential injection, constant infusion)

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However, free iron in mitochondria and the cytoplasm can generate highly oxidative HO, which damages polyunsaturated fatty acids (PUFAs) in the phospholipid bilayer, leading to mitochondrial outer membrane permeabilization (MOMP), ultimately abrogating DNA stability and inducing cell death

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