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(Wu et al., 2019) demonstrated in human umbilical vein ECs and arteries of HHcy mice that Hcy triggers the expression of ER oxidoreductin-1 (Ero1), leading to ER stress and subsequent inflammation

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Following the addition of 200 L of hydroxylamine hydrochloride with a concentration of 1 mM, a reading at 560 nm was taken at time zero

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Triethanolamine Dilute 1:6 with KPE and store on ice