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The cell pellet was underlaid with 1 ml of ice-cold HLB + NS (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 2.5 mM MgCl 2 , 0.5% NP-40, 10% sucrose, 1x phosphatase inhibitor cocktail and 1x protease inhibitor cocktail)

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