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Primary antibodies used were p53 (CST, #9282, 1:1000 dilution), phospho-p53 (CST, #9284, 1:1000 dilution), p21 (CST, #2947, 1:1000 dilution), phospho-histone H2A.X (CST, #9718, 1:1000 dilution), phospho-CHK1 (CST, #2348, 1:500 dilution), phospho-CHK2 (CST, #2661, 1:500 dilution), Vinculin (Santa Cruz, #sc-73614, 1:2000 dilution), alpha-tubulin (CST, #2144, 1:2000 dilution), and beta-actin (Santa Cruz, #sc-47778, 1:2000 dilution)

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Quercetin displayed a dose-dependent inhibitory action: at 50 M, 23% inhibition of both AMV-RT and RAV-2-RT, and at 10 M inhibition of mammalian MMLV-RT of almost 60% were reached (76)

For instance, cationic gold nanoparticles have been shown to be 27 times more cytotoxic than their negative counterparts, due to the disruption of the cell membranes [141-143]

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