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doi: 10.1016/j.crfs.2024.100815 197 akarUoloviMMilenkoviDPagnaccoMMaksimoviJKrstiDet al

Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min