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The following steps were applied in data processing with MZmine 2.53: (i) Mass detection with a noise level of 1000, (ii) Chromatogram builder with a minimum time span of 0.08 min, minimum height of 1000 and a m/z tolerance of 0.006 m/z or 10.0 ppm, (iii) Chromatogram deconvolution using the local minimum search algorithm with a 70% chromatographic threshold, 0.05 min minimum RT range, 5% minimum relative height, 1200 minimum absolute height, a minimum ration of peak top/edge of 1.2 and a peak duration range of 0.085.0, (iv), Isotopic peak grouper with a m/z tolerance of 5.0 ppm, RT tolerance of 0.05 min, maximum charge of 2 and with the most intense isotope set as the representative isotope, (v) Join aligner with a m/z tolerance of 0.009 or 10.0 ppm and a weight for of 2, a RT tolerance of 0.15 min and a weight of 1 and with no requirement of charge state or ID and no comparison of isotope pattern, (vi) Peak list row filter with a minimum of 10% of the samples (vii) Gap filling using the same RT and m/z range gap filler algorithm with an m/z tolerance of 0.009 m/z or 11.0 ppm, (vii) Identification of lipids using a custom database search with an m/z tolerance of 0.008 m/z or 8.0 ppm and a RT tolerance of 0.25 min

This decline can lead to weakness, scarring, fat accumulation inside muscle, and the loss of fast-twitch muscle fibers that are important for quick and powerful movements

Danggui Buxue Tang and Danggui Shaoyao San have been extensively validated in diabetic nephropathy models, exhibiting dual anti-inflammatory and anti-fibrotic properties

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The development of high-resolution multidimensional separation techniques such as high-pressure liquid chromatography coupled with a 16-channel coulometric multi-electrode array system (HPLCCMEAS), can lead to revolutionary changes in our understanding at the molecular level (Matson et al., 1984