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Both compounds are long-acting once-weekly molecules, so protocols are organized around a weekly schedule rather than daily dosing

C6 cells were transfected at 80% confluence with a combination of GluN1 and GluN2A or GluN2B (3.75 g cDNA/wells) + 10 l of Lipofectamine (Invitrogen) diluted in Opti-MEM TM (Gibco TM )

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Nephrol Dial Transplant 32(3):450458 Romagnoli M, Gomez-Cabrera MC, Perrelli MG, Biasi F, Pallard FV, Sastre J, Poli G, Via J (2010) Xanthine oxidase-induced oxidative stress causes activation of NF-kappaB and inflammation in the liver of type I diabetic rats