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It activates key mitophagy regulators, including PTEN-induced kinase 1 (PINK1) and the E3 ubiquitin ligase Parkin, which coordinate the ubiquitination and lysosomal targeting of dysfunctional mitochondria [112]

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A monothiol mechanism was also reported for the in vitro reduction of mammalian RNR 46 and can be also employed, although with a decreased activity, by a Grx-RNR fusion protein from Facklamia ignava 47

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These cumbersome and time-consuming steps have led some manufacturers to develop the direct pay method