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For crystallization, 28 and 20 residues were removed from the N and C termini of the receptor, respectively, and the thermostabilized apocytochrome b 562 RIL (bRIL) protein from Escherichia coli was fused to the deleted N terminus

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Cell Chem Biol 27:365375 Stockwell BR, Friedmann Angeli JP, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascon S, Hatzios SK, Kagan VE et al (2017) Ferroptosis: a regulated cell death nexus linking metabolism, redox biology, and disease