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To purify only RBD we made use of the TEV cleavage site in between the RBD and the GST tag

"Randomized, Controlled Clinical Trial of Zinc Supplementation to Prevent Immunological Failure Inhiv-Infected Adults." Clin Infect Dis 50.12 (2010): 1653-60

& Marsalek, O

10.1038/s41598-020-72635-x 150 MaY.ChenH.JiangY.WangD.AschnerM.LuoW.et al (2024)

subsequently, ASC recruits caspase-1 via filamentous CARD-CARD interactions, which solidifies the condensation and reduces their fluidity, ultimately resulting in the formation of mature inflammasomes (Fig