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We followed the protocol described in Diao et al (2015) by crossing in recombinase and donor transgenes

430 These findings suggest that iron metabolism is abnormal in the cells of Friedreichs Ataxia models, and regulation of iron metabolism may have some ameliorative effects on Friedreichs Ataxia

[24] One of the pathways involves the use of the B vitamins, while the other one uses trimethylglycine (betaine) to help with metabolizing homocysteine

clearly showed that in the presence of millimolar GSH and 40 M O 2 (as in normal tissue), the interaction of NFT with O 2 would outcompete its reaction with GSH by a factor of 10,000 (Miller et al

When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature