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The luminescence of the diluted sample (40 ml) mixed with an equal volume of rL/L reagent (Promega) was measured

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Furthermore, the study defines vitamin C as a required cofactor in collagen synthesis

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Although comparative studies have showed that Hunner lesions patients are older, 86, 87, 101-103 have greater urinary frequency and nocturia, 86, 87, 102, 103 higher ICSI scores, 86, 87, 103 and lower bladder capacity 101, 103 compared to those without Hunner lesion, cystoscopy remains the only reliable way to diagnose the presence or absence of Hunner lesions given the overlaps in clinical symptomatology