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GPX4 enzymatic activity assay Cells were collected in lysis buffer (100 mM Tris pH 7.6, 5 mM EDTA, 1 mM NaN 3 and 0.1% peroxide-free Triton-X100)

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The ion product of water was determined from the same experiment in the pH range 11.4012.00

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doi: 10.1371/journal.pone.0196921 90 YangMChenXHuXLiHHuangHFangYet al

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