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Patients with genetic defects in the gamma-glutamyl cycle

The cell pellet was quickly resuspended in a hypotonic lysis buffer (containing 10 mM HEPES (H109408-5g, Aladdin, Shanghai, China), pH 7.9, 1.5 mM MgCl2 (M434105-100g, Aladdin, Shanghai, China), 10 mM KCl (P433501-250g, Aladdin, Shanghai, China), 0.5 mM DTT (D301909-1ml, Aladdin, Shanghai, China), and washed with it

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Oral supplements can be recommended for some dogs or an injection of cyanocobalamin (usually 1,000 mcg/mL) may be recommended for dogs with severe deficiencies or poor intestinal absorption

ROS can induce mutations in the BRCA1 gene, which is involved in DNA repair, leading to impaired DNA damage response and increased susceptibility to tumorigenesis [100]