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Interestingly, the rate of production is normally higher at basal mitochondrial respiration (state 4) than state 3 in both skeletal muscle and the diaphragm, suggesting that mitochondria might not be the major source of ROS in exercising muscles (Powers and Jackson, 2008

doi: 10.1634/stemcells.2007-0122 207 WilcoxJ

Our results revealed that betaine and DMG levels, as well as the betaine: choline ratio, were reduced in COC users, whereas choline, serine, and the DMG: betaine ratio were significantly increased in COC users compared with controls (Table 4)