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One way, two way or repeated ANOVA and appropriate post-hoc tests were used to identify significant differences, with a significance level set at p 0.9999)

PMID: 39060389 Acta Pharm Sin B 2021 Dec;11(12):4045-4054

Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ

After this, the gelling chambers were removed, and specimens were incubated in 8 U/mL proteinase K (Sigma-Aldrich P2308) in a Tris/EDTA-based digestion buffer (50 mM Tris (pH 8), 25 mM EDTA, 0.5% Triton X-100 and 0.8 M NaCl) at 60C for 4 h

The neural gut-brain axis of pathological protein aggregation in Parkinsons disease and its counterpart in peroral prion infections