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To minimize the time that cells are kept in MAS buffer, just before calibration finished, the cell media was removed, and the cells were washed once with MAS (1) buffer before addition of 180 l MAS (1) buffer

Studies also support that ALA helps lower blood sugar levels

Mutations identified in patients with defective lipoic acid metabolism affect genes involved in the synthesis of iron-sulfur clusters and genes coding for lipoic acid synthetase ( LIAS ), lipoyl transferase 1 ( LIPT1 ), and dihydrolipoamide dehydrogenase (E3 component of -ketoacid dehydrogenase complexes