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Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway

Reactions were initiated by the addition of 10 reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl, 50 mM ATP, 5 mM DTT) and incubated at 37 C for the specified durations

The homogenates were centrifuged (Eppendorf 5810R, Hamburg, Germany) at 1000 g at 4 C for 10 min

Abbreviations 2-AG: 2-Arachidonoylglyerol 5HT 1A : 5-Hydroxytryptamine receptor 1A 5HT 3A : 5-Hydroxytryptamine receptor 3A 17OHP: Progesterone 17-hydroxylase ACOG: American College of Obstetrics and Gynecology ADORA 2A : Adenosine A 2A receptor AEA: Anandamide Caspase: 1-Cysteinyl aspartate protease 1 cAMP: Cyclic adenosine monophosphate CBD: Cannabidiol CBDV: Cannabidivarin CBC: Cannabichromene CBCV: Cannabichromevarin CBG: Cannabigerol CBDA: Cannabidioloic acid CBGA: Cannabigerolic acid CBGV: Cannabigerovarin CBN: Cannabinol CBNA: Cannabinolic acid CB 1 : Cannabinoid receptor type 1 CB 2 : Cannabinoid receptor type 2 CPS: Child Protective Services CYP: Cytochrome p450 enzyme CYP3A5: Cytochrome P450 3A5 CYP3A7: Cytochrome P450 3A7 CYP3A4: Cytochrome P450 3A4 CYP2C9: Cytochrome P450 2C9 CYP1A1: Cytochrome P450 1A1 CYP1A2: Cytochrome P450 1A2 CYP1B1: Cytochrome P450 1B1 CYP2D6: Cytochrome P450 2D6 CYP2C19: Cytochrome P450 2C19 CYP2B6: Cytochrome P450 2B6 CYP2J2: Cytochrome P450 2J2 D2: Dopamine receptors ERK1/2: Extracellular signal-regulated kinase FAAH: Fatty acid amine hydrolase FDA: Food and Drug Administration GPR55: G protein-coupled receptor 55

If Methylcobalamin is left out of the fridge after being mixed it will not damage the medication