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Membranes were blocked for 1 h in protein-free blocking buffer (Thermo), then incubated overnight with 1:1000 diluted anti-GAPDH mouse monoclonal antibody (60004-1-IG

This means the body produces less natural steroid hormones, which can lead to fatigue, weakness, and other systemic symptoms

Accordingly, cells expressing less active GST isoforms are more sensible to GST-metabolized toxins compared to cells with balanced GST activity

Bakaloudi DR, Halloran A, Rippin HL, Oikonomidou AC, Dardavesis TI, Williams J, Wickramasinghe K, Breda J, Chourdakis M

The dataset was randomly split into an 8:2 ratio for training and validation purposes, AUC scores were calculated as our evaluation metric