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For kinetic ( k on and k off ) and thermodynamic (K d ) measurements, single-cycle kinetics were performed at 25 C or 37 C with 1xHBS-EP+ as running buffer

The PDCAAS is a number between 0 And 1 that evaluates protein quality based on its amino acid contents in relation to humans requirements for them

The PFK2 subtype PFKFB3 shows marked overexpression in malignancies including CRC, with its regulation linked to oncogenic activation (e.g., RAS, CDH1) [49]

Adams J, Baral M, Geis E, Mitchell J, Ingram J, Hensley A, Zappia I, Newmark S et al (2009) The severity of autism is associated with toxic metal body burden and red blood cell glutathione levels

Within-group effect sizes (Cohens d ) were based on observed values and calculated by dividing the mean difference with respective standard deviations and correction for the correlation between timepoints