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doi: 10.1016/j.pneurobio.2020.101892

Sulfhydrated sirtuin-1 increasing its deacetylation activity is an essential epigenetics mechanism of anti-atherogenesis by hydrogen sulfide

When GSH was tested together with reduced PfGrx C88S or PfGrx C32S/C88S , the syringe of the stopped-flow spectrofluorometer also contained 0.4 U/mL glutathione reductase (GR) and 0.25 mM NADPH to ensure the removal of trace amounts of GSSG that might interfere with the redox measurements