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Abnormal iron metabolism may cause iron accumulation in various tissues, including the brain, liver and pancreas, potentially inducing neurodegenerative changes such as neuronal loss, gliosis and mitochondrial dysfunction, which may clinically manifest as ataxia, dystonia and dementia (23)

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None of them are introducing a foreign mechanism

We've seen this catastrophic mistake too many times

The vector was linearized using AgeI and BamHI