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Injecting into wet alcohol stings because the alcohol gets carried under the skin by the needle

Cancers 14 , 2919 (2022)

We further investigated whether Ggt1-induced glutamate and its products perturbed downstream pathways

For reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen), 1.2 L of 10 Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 L, and the samples were incubated for 10 min at 95 C