phospho lipase c Structural insights into Phospholipase Cε activity and regulation Structural Insights into Phospholipase C-β

The sonicated lysate was centrifuged and diluted using 10 volumes of cold ChIPdilution buffer (0.01% SDS, 1.1% Triton X100, 1.2 mM EDTA, 16.7 mM TrisHCl, pH 8, 167 mM NaCl)

Subsequently, cells were wounded by pipette tips and washed twice with media to remove detached cells, and photomicrographs of initial wounds were taken using Canon PowerShot A640 camera (at 100 magnification)

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