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Persister Cell Derivation and Treatment Persister cells were derived from colorectal cancer cell lines HCT116 (KRAS G13D ) and SW620 (KRAS G12V ) treated with 5-FU and AZ628, for at least 9 days with fresh drug added every 3 days, and then stained with crystal violet or counted using a Countess Automated Cell Counter (Invitrogen, Carlsbad, CA, USA)

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The piece closed with comments from three more residents, who were unconvinced that the city and state were doing enough to provide safe water: Saterra Hill, 17, a health sciences major at the University of Michigan-Flint, said she and her father purchased several gallon jugs of water each month instead of drinking tap water

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Functional role and specificity The study of structural plasticity of glutamatergic synapses has focused on the relationship between morphological parameters of dendritic spines and physiological properties of its excitatory synapse (Nimchinsky et al., 2002