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55 Integrative bioinformatics and experimental analysis revealed that TEAD can serve as a novel prognostic target for HCC, and that knockdown of TEAD2 induces ferroptosis through iron accumulation and subsequent oxidative damage

Briefly, 2 g plasmid, 4 L P3000 and 4 L Lipofectamine 3000 were mixed into 500 L Opti-MEM medium in order for 30 min, then added up to 3 mL with DMEM medium before transferring to 6-well plate

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J Child Adolesc Psychopharmacol 2014;24:525-7

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