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Preparation Condition 2 (EDTA deproteinization 80 o C): Anticoagulants used during collection impacts measures of GSH Suggesting the anticoagulant used during sample collection significantly alters plasma GSH, we found lower levels of free GSH, higher levels of GSSG, and a lower ratio of free GSH to GSSG in the heparinized plasma (Preparation Condition 1) compared to the EDTA-treated plasma (Preparation Condition 2)

A randomized, double-blind study from King Chulalongkorn Memorial Hospital found that participants taking 500mg of oral glutathione daily showed decreased melanin indices at all six measured sites after just 4 weeks