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Absence of CBS would preclude synthesis of cystathionine in the malaria parasite

The standard curve was drawn against the standard samples, and the relative glutamate, glycine and cysteine level in the samples was calculated by the protein concentration of the samples

However, in the absence of evidence for cysteinylation of KEAP1 in cells, we cannot completely exclude the possibility that Cys signaling is mediated indirectly

For PNGase H+ application, exchanging with distilled water was omitted, and slides were immediately dried in a desiccator after antigen retrieval to ensure acidic conditions necessary for optimal PNGaseH+ activity

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