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Loss of HIF1A from pancreatic cancer cells increases expression of PPP1R1B and degradation of p53 to promote invasion and metastasis

Therefore, inhibition or induction of iron decreases can be achieved by overexpression or knockout of NCOA4

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Lysis solution was prepared with Nuclei PURE lysis buffer (NUC-201, Sigma-Aldrich), 1 mM dithiothreitol (D9779, Sigma-Aldrich), and 0.1 % Triton X-100 (NUC-201, Sigma-Aldrich) according to manufacturer protocol, and an RNase inhibitor mix (0.04 U/L SUPERaseIN RNase Inhibitor [AM 2696, Thermo Fisher]