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doi: 10.1016/j.applanim.2006.11.008

Sorted single-cell suspensions were resuspended at an estimated final concentration of 1000 cells/l and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM)

With the development of targeted drugs and combination therapy strategies, preclinical studies and clinical trials on the targeted intervention of Wnt/-catenin signal transduction in malignant tumours have been gradually performed and are promising candidates for individualized therapy in cancer patients

All samples obtained by the Blood Service Biobank were in frozen 200 ul aliquots, anonymized, coded and accompanied with gender, age, height and weight information

They can vary in size from a few nanometers to tens of micrometers