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Lysophosphatidic acid induces podocyte pyroptosis in diabetic nephropathy by an increase of egr1 expression via downregulation of ezH2

Membranes were blocked with 5% bovine serum albumin in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at room temperature, then incubated overnight at 4 C with primary antibodies (phospho-FoxO3a, FoxO3a, MuRF1, and Atrogin-1)