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Going forward, additional research could include larger, long-term clinical trials to determine if peptide therapy benefits extend beyond AMH numbers, like supporting live birth rates or overall ovarian health

Luciferase assay PGL6-VGF-Luc or PGL6-gcg-Luc plasmids and Renilla luciferase expression plasmids were co-transfected into TC1-6 cells using Lipofectamine 2000 (Thermo Fisher, 11668), according to the manufacturers instructions

Image analysis based on fluorescence intensity was performed as described in our previous study [24]

A meta-analysis to elucidate the main impacts and extent of RPM on dairy cows performance (Patton, 2010) gathered a total of 129 studies and allowed 75 dietary comparisons between the control and RPM-enriched diets