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Journal of Nanobiotechnology, 18 (1)

For analysis of flavonoids by HPLCMS, reaction volumes were split after the plate reader incubations, and a 40 l aliquot was extracted with 80 l of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 L aliquot was extracted with 80 l of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids

However, on the long term or under specific pathological conditions, it can have detrimental effects on the brain and the BBB [120]

CHAC1 amplifies this oxidative cascade by degrading residual GSH, further elevating ROS levels and preventing GPX4-mediated detoxification

The GSH-GSSG ratio is an important indicator of the efficiency of our metabolism and the amount of cellular oxidative stress